Effectiveness of secondary metabolites fungi Lecanicillium saksenae for Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) in laboratory

Abstract

Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) has successfully invaded and spread to almost all provinces in Indonesia which can have a significant impact on corn production. This study aims to determine the effectiveness of secondary metabolites of Lecanicillium saksenae on feeding ability and growth period of S. frugiperda. This study used a factorial Randomized Block Design (RCBD) consisting of two factors consisting of 10 treatment combinations. The first factor is the concentration of secondary metabolites of L. saksenae consisting of a concentration of 0% (control) (K0), 10% (K1), 20% (K2), 30% (K3), and synthetic insecticides (with active ingredients emamectin benzoate and lufenuron) 1 ml/L (K4). The second factor is the application method consisting of the spraying method on the larvae (A1) and feed dipping (A2). Each treatment was repeated three times so that there were 30 experimental units. Data were analyzed using the F test with a 5% error rate, if it showed a significant difference, then it was further tested using DMRT at a 5% error rate. The results showed that secondary metabolites of L. saksenae at a concentration of 30% with the method of spraying on larvae and dipping feed caused decreased feeding ability of 36.71% and 37.89%, and inhibited the growth of larvae 39.73% and 37.90%, pupae 82.91% and 83.35%, and imago 84.54% and 71.52%.